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Custom iPSC Generation Packages
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Complete Characterization Package includes: Mycoplasma Testing, AP Staining, ICC for Pluirpotency Markers, G-Band Karyotype, RT-qPCR for Endogenous Pluripotency gene expression, hPSC Scorecard Assay, EBNA1 gDNA PCR, Cell Line Authentication (STR)

Essential Characterization Package includes: Mycoplasma Testing, AP Staining, ICC for Pluripotent Markers, G-Band Karyotype, Cell Line Authentication (STR)

Source tissues can include: fibroblast (skin), fresh whole blood (PBMCs), MSCs, adipose cells, various stromal epithelial cell types, and lymphoblastoid cells lines (LCLs or EBV B-cells). The fresh blood must be collected in CPT tubes and centrifuged according to manufacturer's directions. All reprogramming is preformed using non-integrating techniques in defined media (serum and KSR-free) and feeder-free conditions (no MEFs). Cost for each patient line includes the expansion and banking of three thoroughly characterized clonal lines under feeder-free conditions. Each cell line will be accompanied by a rigorous Certificate of Analysis to ensure pluripotency, normal karyotype, embryoid body formation and a variety of services related to the characterization of the pluripotent state (see below).

Pluripotent Stem Cell (PSC) Quality Control and Characterization Services
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Biobanking and PSC Thawing

As part of the custom iPSC generation service, the core will expand and bank three fully characterized iPSC clones per cell line. This service is also available to iPSC and ESC lines generated outside of Cedars-Sinai.

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PBMC Isolation and Processing
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Browse Cell Line Catalog

Hundreds of already established lines spanning over 30 degenerative diseases.