Protocols

Analysis

Cell Concentration

  • Approximately 1 x 10^6 cells per mL in PBS containing 1% BSA. No phenol red should be used. Minimum sample volume recommended is 500 uL.
Sorting

Cell Concentration

  • Approximately 20-25 x 10^6 cells per mL resuspended in PBS containing 1% BSA. No phenol red should be used. Minimum sample volume is 500 uL.
  • For single cell sorting into 96-well plates, cell concentration should be 1-5 x10^6 cells per mL.
Filter sample

Have Questions or Need Help?

Contact us if you have questions or would like to learn more about the Flow Cytometry Core at Cedars-Sinai.

Cedars-Sinai Flow Cytometry Core
8700 Beverly Blvd.
Davis Building, Room 4029
Los Angeles, CA 90048